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      Cell Biolabs彗星实验试剂盒

      由于环境因素和细胞内的正常代谢过程造成的DNA损伤,每个细胞每天都会发生1,000到1,000,000个。虽然这些只占人类基因组约60亿个碱基中的一小部分,但如果关键基因损伤未及时修复,可能会阻碍细胞的正常生理功能,进而增加癌变可能。彗星实验,或称单细胞凝胶电泳(Single cell gel electrophoresis,SCGE),是一种测量单个细胞DNA损伤的常用技术。其原理很简单,即在电泳场中,将受损细胞DNA(包含片段和链断裂)与完整的DNA分离,通过显微镜可观察到损伤细胞呈现出典型的彗星状尾巴,然后通过测量计算彗尾大小对比出细胞DNA损伤的程度。因为彗星实验的特点,该方法几乎被用来评估任何类型的真核细胞的 DNA 修复能力,包括双、单链断裂的不同的 DNA 损伤情况。是一种能快速、大通量检测真核细胞DNA损伤进而判别遗传毒性的技术。 彗星实验结果图 彗星实验原理虽简单,但操作繁琐,需要丰富的实验经验和技巧,尤其常常出现的“脱胶”问题,困扰了许多科研人员。除此之外,有时为了跑出完美的“彗星”图案放在paper里,还需要重复做许多次实验,费时费力。为了解决上述问题,我们推荐CellBiolabs的OxiSelectTMComet Assay Kit即彗星实验试剂盒来检测细胞的DNA损伤。该试剂盒不仅能让彗星实验化繁为简,还有两种不同规格(3孔和96孔)的细胞电泳凝胶板供选择,让少量样本和大量样本的DNA损伤检测通通轻松hold住。用该试剂盒做彗星实验流程如下图。 除了操作简便,OxiSelectTMComet Assay Kit还有以下优点: 1) 适用于各种DNA损伤检测,是一款非常好用的DNA损伤检测筛选工具; 2) 试剂盒中的载玻片经过特殊处理以粘附低熔点琼脂糖,避免“脱胶”问题出现; 3) 采用特殊的DNA荧光染料,能有效降低背景干扰,更加方便读取实验结果。 彗星实验试剂盒信息:品名 货号 规格 说明 OxiSelectTM Comet Assay Kit (3-Well Slides) STA-350 15 assays 试剂盒内有5张3孔载玻片和彗星实验所需的低熔点琼脂糖、裂解液及DNA荧光染料等,共可检测15个样品。 OxiSelectTM Comet Assay Kit (3-Well Slides) STA-351 75 assays 试剂盒内有25张3孔载玻片和彗星实验所需的低熔点琼脂糖、裂解液及DNA荧光染料等,共可检测75个样品。 OxiSelectTM Comet Assay Kit (96-Well Slides) STA-355 96 assays 试剂盒内有1张96孔载玻片和彗星实验所需的低熔点琼脂糖、裂解液及DNA荧光染料等,共可检测96个样品。 为了满足客户更多样的实验需求,彗星实验试剂盒内特殊处理电泳载玻片还可以单独购买,详情如下: 品名 货号 规格 产品图片 Comet Assay Slides, 3-Well STA-352 5 slides STA-353 25 slides Comet Assay Slides, 96-Well STA-356 1 slides STA-356-5 5 slides 产品部分发表文献: Maiuri, T. et al. (2016). Huntingtin is a scaffolding protein in the ATM oxidative DNA damage response complex. Hum. Mol. Genet. doi:10.1093/hmg/ddw395. Irianto, J. et al. (2016). Nuclear constriction segregates mobile nuclear proteins away from chromatin. Mol. Bio. Cell doi:10.1091/mbc.E16-06-0428. Andronescu, E. et al. (2016). Nanomaterials for medical applications: Benefits and risks. J Nanomater. doi:10.1155/2016/8284319. Liu, Z. et al. (2016). Canonical microRNAs enable differentiation, protect against DNA damage, and promote cholesterol biosynthesis in neural stem cells. Stem Cells and Dev. doi:10.1089/scd.2016.0259. Dai, C. et al. (2016). Curcumin ameliorates furazolidone-induced DNA damage and apoptosis in human hepatocyte L02 cells by inhibiting ROS production and mitochondrial pathway. Molecules. 21:1061. Beegle, J. R. et al. (2016). Preclinical evaluation of mesenchymal stem cells overexpressing VEGF to treat critical limb ischemia. Mol Ther Methods Clin Dev.doi:10.1038/mtm.2016.53. Suzuki, Y. et al. (2016). Pharmacodynamics of anti-inflammatory drugs–intranasal corticosteroid and dna damage. Pharmacodynamics of anti-inflammatory drugs. 117-126. Zhang, J. et al. (2016). Inhibition of Glucose-6-Phosphate Dehydrogenase could Enhance 1, 4-Benzoquinone-induced Oxidative Damage in K562 Cells. Oxid Med Cell Longev. doi:10.1155/2016/3912515. Jang, J. H. et al. (2016). APO-9′-fucoxanthinone extracted from undariopsis peteseniana protects oxidative stress-mediated apoptosis in cigarette smoke-exposed human airway epithelial cells. Mar Drugs. doi:10.3390/md14070140. Ebeid, S. A. et al. (2016). Assessment of the radioprotective effect of propolis in breast cancer patients undergoing radiotherapy. New perspective for an old honey bee product. J Radiat Res Appl Sci. doi:10.1016/j.jrras.2016.06.001. Wang, H, & Kim, N. H. (2016). CDK2 is required for the DNA damage response during porcine early embryonic development. Biol Reprod. doi:10.1095/biolreprod.116.140244. Zhao, X. et al. (2016). Dioscin induces apoptosis in human cervical carcinoma HeLa and SiHa cells through ROS-mediated DNA damage and the mitochondrial signaling pathway. Molecules. doi:10.3390/molecules21060730. Chen, X. et al. (2016). Zidovudine, abacavir and lamivudine increase the radiosensitivity of human esophageal squamous cancer cell lines. Oncol Rep. 36:239-246. Coleman, J. et al. (2016). Detecting Apoptosis, Autophagy, and Necrosis. Apoptosis Methods in Toxicology . doi:10.1007/978-1-4939-3588-8_5. Ding, Y. et al. (2016). Induction of ROS overload by alantolactone prompts oxidative DNA damage and apoptosis in colorectal cancer cells. Int J Mol Sci. doi:10.3390/ijms17040558. Cui, F. M. et al. (2016). The role of miR-34a in tritiated water toxicity in human umbilical vein endothelial cells. Dose-Response. doi:10.1177/1559325816638585. Laks, D. R. et al. (2016). Inhibition of nucleotide synthesis targets brain tumor stem cells in a subset of glioblastoma. Mol Cancer Ther. doi:10.1158/1535-7163.MCT-15-0982. Abubakar, I. B. et al. (2016). Synergistic cytotoxic effects of combined δ-tocotrienol and jerantinine B on human brain and colon cancers. J Ethnopharmacol. doi:10.1016/j.jep.2016.03.004. Hofstetter, C. et al. (2016). Inhibition of KDM6 activity during murine ESC differentiation induces DNA damage.J Cell Sci. 129:788-803. Si, L. et al. (2016). Dioscin suppresses human laryngeal cancer cells growth via induction of cell-cycle arrest and MAPK-mediated mitochondrial-derived apoptosis and inhibition of tumor invasion. Eur J Pharmacol. doi:10.1016/j.ejphar.2016.02.009. Qu, L. et al. (2016). Corosolic acid analogue, a natural triterpenoid saponin, induces apoptosis on human hepatocarcinoma cells through mitochondrial pathway in vitro. Pharm Biol. doi:10.3109/13880209.2015.1104699. Singh, A. K. et al. (2015). Parental age affects somatic mutation rates in the progeny of flowering plants. Plant Physiol. doi:10.1104/pp.15.00291. Yuan, L. et al. (2015). Serum collected from fruit and vegetable juice treated rats antagonizing H2O2-induced oxidative damage in PC12 cells. J Funct Foods. 20:496-505. Ramy, N. et al. (2015). Jaundice, phototherapy and DNA damage in full-term neonates. J Perinatol. doi:10.1038/jp.2015.166. Wu, C. F. et al. (2015). Anticancer activity of cryptotanshinone on acute lymphoblastic leukemia cells. Arch Toxicol. doi:10.1007/s00204-015-1616-4. Kim, M. J. et al. (2015). Antibacterial effect and mechanism of high-intensity 405±5nm light emitting diode on Bacillus cereus, Listeria monocytogenes, and Staphylococcus aureus under refrigerated condition. J Photochem Photobiol B. 153:33-39. Wang, X. et al. (2015). Enhancement of arabinocytosine (AraC) toxicity to AML cells by a differentiation agent combination. J Steroid Biochem Mol Biol. doi:10.1016/j.jsbmb.2015.08.023. Sun, X. et al. (2015). Electrochemical detection of 8-hydroxy-2′-deoxyguanosine as a biomarker for oxidative DNA damage in HEK293 cells exposed to 3-chloro-1, 2-propanediol. Anal Methods.doi:10.1039/C5AY01246E. Neumann, J. et al. (2015). Mangrove dolabrane‐type of diterpenes tagalsins suppresses tumor growth via ROS‐mediated apoptosis and ATM/ATR–Chk1/Chk2‐regulated cell cycle arrest. Int J Cancer. doi: 10.1002/ijc.29629. Singh, A. K. et al. (2015). Parental age affects somatic mutation rates in the progeny of flowering plants. Plant Physiol. 168:247-257. Hou, W. et al. (2015). The protecting effect of deoxyschisandrin and schisandrin B on HaCaT cells against UVB-induced damage. PLoS One. 10:e0127177. Kim, M. J. et al. (2015). Inactivation by 405±5 nm light emitting diode on Escherichia coli O157: H7, Salmonella Typhimurium, and Shigella sonnei under refrigerated condition might be due to the loss of membrane integrity. Food Control. doi:10.1016/j.foodcont.2015.05.012. Haeger, S. M. et al. (2015). Smad4 loss promotes lung cancer formation but increases sensitivity to DNA topoisomerase inhibitors. Oncogene. doi: 10.1038/onc.2015.112. Ong, J. Y. et al. (2015). 2-Methoxy-1, 4-naphthoquinone (MNQ) induces apoptosis of A549 lung adenocarcinoma cells via oxidation-triggered JNK and p38 MAPK signaling pathways. Life Sci. doi: 10.1016/j.lfs.2015.03.019. Prasad, M. A. et al. (2015). Ebf1 heterozygosity results in increased DNA damage in pro-B cells and their synergistic transformation by Pax5 haploinsufficiency. Blood. doi: 10.1182/blood-2014-12-617282. Obstoy, B. et al. (2015). Safety and performance analysis of acriflavine and methylene blue for in vivo imaging of precancerous lesions using fibered confocal fluorescence microscopy (FCFM): an experimental study. BMC Pulm Med. 15:30. Xiong, J. et al. (2015). Stemness factor Sall4 is required for DNA damage response in embryonic stem cells.J Cell Biol. 208:513-520. Hu, Y. et al. (2015). Bile acids regulate nuclear receptor (nur77) expression and intracellular location to control proliferation and apoptosis. Mol Cancer Res. 13:291-292. Gong, L. et al. (2015). p53 isoform Δ113p53/Δ133p53 promotes DNA double-strand break repair to protect cell from death and senescence in response to DNA damage. Cell Res. 25:351-369. Jin, L. et al. (2015). Association between oxidative DNA damage and the expression of 8-oxoguanine DNA glycosylase 1 in lung epithelial cells of neonatal rats exposed to hyperoxia. Mol Med Rep. doi: 10.3892/mmr.2015.3339. 北京西美杰科技有限公司是Cell Biolabs品牌全国一级代理,为用户提供完善的技术支持与售后服务。如对产品感兴趣欢迎拨打西美杰客服热线400-050-4006或了解更多信息。 更多>

      给药“神器”Alzet渗透压泵,让长时间给药实验更加简单高效

      Alzet渗透压泵不仅可以保证持续稳定的输出药物,还能实现超长时间的给药实验,被广泛应用于多个研究领域,并在国际高水平期刊上发表文献多达19,000余篇。北京西美杰科技有限公司是Alzet品牌中国一级代理,可为Alzet用户提供全方位的售前售后技术问题解答。 更多>

      新品| Mirus升级版CHO细胞高滴度瞬转表达系统

      CHO细胞是生产抗体类药物广泛应用的工程细胞,而CHO细胞瞬时转染是新药研发过程中必不可少的技术手段。为了用低成本在较短时间内表达出足量的目的蛋白,Mirus新推出了一款适用于悬浮CHO细胞瞬转和蛋白高滴度表达的平台——CHOgro? High Yield Expression System。相比于Mirus第一代CHOgro?表达系统,新版CHOgro?高滴度蛋白表达系统引入了效价增强剂(CHOgro? Titer Enhancer)组分,在确保操作流程精简的同时,能够大幅度提高瞬转CHO细胞的蛋白产量。 更多>

      NEW!专利产品Bambanker无血清即用型细胞冻存液

      Bambanker无血清即用型细胞冻存液—给细胞更多的呵护 专利产品(专利号1347040) Serum-free for sensitive cell lines, like ES and primary cells, Gradual or programmable freezing is no longer necessary BAMBANKER产品特点 即用型细胞冻存液,无需程序性降温 尤其适用于各类敏感细胞,如ES细胞、原代细胞 高复苏率(请见对比图效果) 可-80℃或液氮中长期冻存,稳定保存1年以上 无血清,成份确定,避免由支原体、病毒、朊病毒及其他病毒颗粒引发的污染 产品经无菌检测,符合日本药典 产品可2~8 ℃稳定保存2年 BAMBANKER操作流程使用说明: 收集对数生长期的细胞(5 x 105 ~ 1 x 107) 用1ml BambankerTM重悬细胞,置于冻存管中,无需预冷。 品名 货号 规格 BambankerTM BB01 120ml BAMBANKER系列冻存液: 品名 描述 货号 规格 BambankerTM Direct 细胞冻存时无需离心,非常适用于杂交瘤细胞 BBD01 20ml BambankerTM HRM 使用人血清白蛋白,无动物源性 BBH01 20ml BAMBANKER is manufactured by LYMPHOTEC Inc. BAMBANKER与其他相关产品的复苏率效果比较 Bambanker使用发表文献(部分) ü T. Hikichi et al., Differentiation potential of parthenogenetic embryonic stem cells is improved by nuclear transfer. Stem cells25, 46 (Jan, 2007). ü S. Mieno et al., Characteristics and function of cryopreserved bone marrow-derived endothelial progenitor cells. The Annals of thoracic surgery85, 1361 (Apr, 2008). ü S. Hatoya et al., Effect of co-culturing with embryonic fibroblasts on IVM, IVF and IVC of canine oocytes. Theriogenology66, 1083 (Sep 15, 2006). ü D. Liu et al., Relation between human decay-accelerating factor (hDAF) expression in pig cells andinhibition of human serum anti-pig cytotoxicity: value of highly expressed hDAF for xenotransplantation. Xenotransplantation14, 67 (Jan, 2007). ü S. K. Zaidi et al., Runx2 deficiency and defective subnuclear targeting bypass senescence to promote immortalization and tumorigenic potential. Proceedings of the National Academy of Sciences of the United States of America104, 19861 (Dec 11, 2007). ü S. Mieno et al., Effects of diabetes mellitus on VEGF-induced proliferation response in bone marrow derived endothelial progenitor cells. Journal of cardiac surgery25, 618 (Sep, 2010). ü Y. Shimizu et al., Impaired Tax-specific T-cell responses with insufficient control of HTLV-1 in a subgroup of individuals at asymptomatic and smoldering stages. Cancer science100, 481 (Mar, 2009). 更多>

      Cali-Bio琼脂糖Hg agarose低电内渗高分辨率低熔点

      美国Cali-Bio公司新系列高品质琼脂糖 NO.1 HgAgarose LE低电内渗琼脂糖(绿色优选) 简介:HgAgarose LE高纯度低电内渗(Low EEO)多用途琼脂糖,是“绿色”琼脂糖,采用绿色环保原创工艺,生产全程不使用有机